Saccharomyces cerevisiae polo-like kinase, Cdc5 exhibits ATP-dependent Mg2+-enhanced kinase activity in vitro
نویسندگان
چکیده
منابع مشابه
Cloning, expression and purification of functionally active Saccharomyces cerevisiae Polo-like Kinase, Cdc5 in E. coli
Article history: Received on: 12/06/2015 Revised on: 30/06/2015 Accepted on: 26/07/2015 Available online: 24/08/2015 Polo-like Kinases (PLKs) belong to the serine/threonine family of protein kinases. They govern cell cycle progression in concert with CDKs and other cell cycle kinases in most eukaryotes studied. Polo-box domain, the signature motif of PLKs, functions to recruit the kinase to its...
متن کاملGeneration of an inducible system to express polo-like kinase, Cdc5 as TAP fusion protein during meiosis in Saccharomyces cerevisiae
Tandem affinity purification (TAP) is a highly efficient method for isolation of protein complexes from endogenous biological macromolecules. TAP system consists of dual affinity tags that facilitates the sequential purification of the desired proteins expressed at their low levels in vivo. Polo-like kinases (PLK) are serine/threonine protein kinases that are the crucial regulators of cell cycl...
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Polo kinase is activated as cells enter mitosis and plays a central role in coordinating diverse mitotic events, yet the mechanisms leading to activation of Polo kinase are poorly understood . Work in Xenopus meiotic cell cycles has suggested that Polo kinase functions in a pathway that helps trigger activation of Cdk1 . However, studies in other organisms have suggested that activation of Polo...
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During meiosis, two consecutive rounds of chromosome segregation yield four haploid gametes from one diploid cell. The Polo kinase Cdc5 is required for meiotic progression, but how Cdc5 coordinates multiple cell-cycle events during meiosis I is not understood. Here we show that CDC5-dependent phosphorylation of Rec8, a subunit of the cohesin complex that links sister chromatids, is required for...
متن کاملCell cycle regulation of the Saccharomyces cerevisiae polo-like kinase cdc5p.
Progression through and completion of mitosis require the actions of the evolutionarily conserved Polo kinase. We have determined that the levels of Cdc5p, a Saccharomyces cerevisiae member of the Polo family of mitotic kinases, are cell cycle regulated. Cdc5p accumulates in the nuclei of G2/M-phase cells, and its levels decline dramatically as cells progress through anaphase and begin telophas...
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ژورنال
عنوان ژورنال: Heliyon
سال: 2019
ISSN: 2405-8440
DOI: 10.1016/j.heliyon.2019.e03050